I. Molecular laboratory
II. Human cell culture laboratory
III. Animal cell culture laboratory
IV. Viral cell culture laboratory
V. Coronavirus diagnosis laboratory at Abu Ali Sina Transplant Hospital in Shiraz
VI. Cytomegalovirus diagnostic laboratory at Abu Ali Sina Transplant Hospital in Shiraz
VII. Liver and pancreas cell isolation laboratory at Shiraz Abu Ali Sina Transplant Hospital
Research Facilities
Shiraz Transplant Research Center mostly welcome to collaborate with all departments and research centers in the university and around the country.
Following is a brief description of the main facilities currently available in the center.
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Facilities |
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Bio-bank
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DNA and serum samples bank of transplant patients Amnion bank: cryo-preserved amnion membrane for usage as a biologic dressing |
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General equipments |
Nanodrop: evaluation of DNA, RNA, and protein concentrations Microplate spectrophotometer: for absorbance measurements Ph. Meter: evaluation of the liquid agents pH Low- temperature freezers (-20 and -80 c): for the storage of materials and samples Ice- maker: for producing ice |
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Cell laboratory |
Main laboratory |
Flowcytometry: sorter for cell characteristic analyzing Electrospinning device: to create electrospun fibers in tissue engineering Upright microscope: for slide observation Biosafety cabinets: for working on animals and staining the samples Centrifuge: to concentrate cells and separate them from old growth media Hot-plate: for heating samples in glass beakers, vials, bottles and other vessels Vortex: for mixing laboratory samples in test tubes, well plates, or flasks Sonicator: applying sound energy to agitate particles in a sample |
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Cell and tissue culture rooms |
Co2 incubators: to maintain an optimal environment for cell growth Laminar flow hood: for cell and tissue culture Nitrogen tank: for cell freezing Refrigerator: for the storage of materials Inverted microscope: to observe the cells Fluorescent microscope: to image specific features of Stereo microscope: for low-magnification applications Water bath: heat source for heating the samples or materials |
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Molecular laboratory |
Main laboratory |
Thermal cycler: to amplify segments of DNA Shaker: for mixing laboratory samples Vortex: for mixing laboratory samples in test tubes, well plates, or flasks Dry bath: to thaw, boil, incubate and warm samples Dry block: to uniformly and simultaneously heat or cool an entire block of test tubes or reaction vials Gene-Pulser: to produce controlled exponential or square wave electrical pulses for cell electroporation Safety cabinets: For isolation of DNA or RNA, cDNA synthesis, preparation of PCR mix Centrifuges: to separate different phase of a mixture Applied biosystems real-time PCR instrument: to amplify segments of DNA or cDNA Laboratory scale: for determining the weight or mass Refrigerators and Low-temperature freezers: for the storage of materials |
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Cell culture room |
Co2 incubators: to maintain an optimal environment for cell growth Laminar flow hood: for virous culture Refrigerator: for the storage of materials Inverted microscope: to observe the samples Water bath: heat source for heating the samples or materials Nitrogen tank: for cell freezing |
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